Fischer peptide synthesis

What is Fischer peptide synthesis?

The Fischer peptide synthesis involves the creation of polypeptides through a series of steps. Initially, an α-chloro or α-bromo acyl chloride is combined with an amino acid ester. This mixture is then subjected to hydrolysis to form an acid, which is subsequently transformed into a new acid chloride.

Fischer peptide synthesis - general reaction scheme
Fischer peptide synthesis

This acid chloride is then condensed with another amino acid ester, and the process repeats with additional amino acids. Finally, the terminal chloride is converted into an amino group with the addition of ammonia.


Fischer, E. (1903), Synthese von Polypeptiden. [Synthesis of polypeptides] Ber. Dtsch. Chem. Ges., 36: 2982-2992.