Merrifield solid-phase peptide synthesis

What is Merrifield solid-phase peptide synthesis?

Merrifield solid-phase peptide synthesis (SPPS) is a method used for synthesizing lengthy peptides. This process involves the following steps:

  1. Attaching the C-terminal amino acid to a polymeric support resin that is insoluble.
  2. Extending the peptide chain.
  3. Cleavaging the peptide from the resin through cleavage.
Merrifield solid-phase peptide synthesis

References

Solid Phase Peptide Synthesis. I. The Synthesis of a Tetrapeptide
R. B. Merrifield
Journal of the American Chemical Society 1963 85 (14), 2149-2154
DOI: 10.1021/ja00897a025

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