Peptide (amide) coupling

What is Peptide (Amide) coupling?

Peptide coupling, also known as amide coupling, is an organic chemical reaction in which two amino acids are joined together through a peptide bond to form a dipeptide or longer peptide. The reaction is an important step in the synthesis of proteins and is used in a variety of applications, including the production of pharmaceuticals, food additives, and other chemicals..

There are several different methods for performing peptide coupling, including the Merrifield synthesis, which involves the use of a solid support to synthesize peptides in a stepwise manner, and the Fmoc (9-fluorenylmethoxycarbonyl) method, which uses a protecting group to control the regiochemistry of the reaction..

One of the key challenges in peptide coupling is controlling the regiochemistry of the reaction, as the amino acids can be joined together in different configurations, resulting in different peptides. In addition, the reaction can be sensitive to the presence of impurities, such as water and oxygen, which can interfere with the reaction and decrease the yield..


Peptide coupling is an important tool for synthesizing proteins and has a wide range of applications in the fields of chemistry and biochemistry..